Aggarwal B B, Farmer S W, Papkoff H, Stewart F, Allen W R
Endocrinology. 1980 Jun;106(6):1755-9. doi: 10.1210/endo-106-6-1755.
Equine chorionic gonadotropin (eCG) secreted by horse trophoblast cells cultivated in vitro was isolated and its chemical, immunochemical, and biological characteristics were compared to those of the gonadotropin secreted in vivo and isolated from PMS. It was also compared with eCG isolated from the tissue of origin, the endometrial cups. The gonadotropin secreted in vitro had a smaller molecular size, contained appreciably less carbohydrate, and showed different amino-terminal residues from that secreted in vivo. In addition, there were significant differences in amino acid composition between eCG secreted in vivo and that secreted in vitro. The reactivity of eCG (isolated from the medium of cultured trophoblast cells) in homologous eCG (isolated from PMS) and equine LH and FSH RIAs suggested close antigenic similarities to eCG isolated from PMS. However, compared to serum-derived eCG, eCG secreted in vitro showed reduced activity in both LH (13%) and FSH (9-24%) bioassays and in LH and FSH radioreceptor assays (40-50%). The differences in bioassay potencies may be accounted for in part by the lower sialic acid content of the gonadotropin secreted in vitro. With respect to both bioactivity and chemical composition, the eCG secreted in vitro more closely resembled the eCG isolated from endometrial cups than that isolated from serum.
对体外培养的马滋养层细胞分泌的马绒毛膜促性腺激素（eCG）进行了分离，并将其化学、免疫化学和生物学特性与体内分泌并从孕马血清（PMS）中分离出的促性腺激素的特性进行了比较。还将其与从起源组织子宫内膜杯分离出的eCG进行了比较。体外分泌的促性腺激素分子尺寸较小，碳水化合物含量明显较少，并且显示出与体内分泌的促性腺激素不同的氨基末端残基。此外，体内分泌的eCG与体外分泌的eCG在氨基酸组成上存在显著差异。eCG（从培养的滋养层细胞培养基中分离）在同源eCG（从PMS中分离）以及马促黄体生成素（LH）和促卵泡激素（FSH）放射免疫分析中的反应性表明，它与从PMS中分离出的eCG具有密切的抗原相似性。然而，与血清来源的eCG相比，体外分泌的eCG在LH生物测定（13%）和FSH生物测定（9 - 24%）以及LH和FSH放射受体测定（40 - 50%）中均显示活性降低。生物测定效力的差异可能部分归因于体外分泌的促性腺激素较低的唾液酸含量。就生物活性和化学成分而言，体外分泌的eCG与从子宫内膜杯分离出的eCG比与从血清中分离出的eCG更为相似。
