Lopez P, Perez Ureña M T, Garcia E, Espinosa M
J Bacteriol. 1980 Apr;142(1):229-35. doi: 10.1128/jb.142.1.229-235.1980.
Glucosylated and nonglucosylated bacteriophage T4 deoxyribonucleic acids (DNAs) are able to bind to competent cells of Bacillus subtilis, although the former does so in a rather unstable fashion, probably because of the glucosylation. Several heterologous DNAs compete with homologous DNA for the same receptors in binding and in transformation. A different pattern in competition for DNA binding was observed for homologous and T4 glucosylated DNAs in intact cells as compared with protoplasts or membrane vesicles. The results are consistent with the existence of two types of receptor sites on the membrane of competent B. subtilis cells.
糖基化和非糖基化的噬菌体T4脱氧核糖核酸(DNA)能够与枯草芽孢杆菌的感受态细胞结合,尽管前者的结合方式相当不稳定,这可能是由于糖基化的缘故。几种异源DNA在结合和转化过程中与同源DNA竞争相同的受体。与原生质体或膜泡相比,在完整细胞中观察到同源DNA和T4糖基化DNA在竞争DNA结合方面存在不同模式。这些结果与枯草芽孢杆菌感受态细胞的膜上存在两种类型的受体位点相一致。