Joenje H, Konings W N, Venema G
J Bacteriol. 1975 Mar;121(3):771-6. doi: 10.1128/jb.121.3.771-776.1975.
Competent cultures of Bacillus subtilis 168 have been fractionated into a high-competent and a low-competent fraction by a large-scale separation technique. Membrane vesicles isolated from both cell fractions are equally active in the transport of L-glutamate. Both membrane vesicle preparations seem to have similar endo- and exonuclease activities. Also, both preparations are capable of binding deoxyribonucleic acid. However, especially at low deoxyribonucleic acid concentrations (1 mug or less per ml), vesicles obtained from competent cells bind significantly more deoxyribonucleic acid (up to sixfold) than vesicles from noncompetent cells.
枯草芽孢杆菌168的感受态培养物已通过大规模分离技术分为高感受态部分和低感受态部分。从这两个细胞部分分离出的膜囊泡在L-谷氨酸转运方面具有同等活性。两种膜囊泡制剂似乎具有相似的内切核酸酶和外切核酸酶活性。此外，两种制剂都能够结合脱氧核糖核酸。然而，特别是在低脱氧核糖核酸浓度（每毫升1微克或更低）下，从感受态细胞获得的囊泡比非感受态细胞的囊泡结合的脱氧核糖核酸明显更多（高达六倍）。
