Yarmush M L, Mandy W J, Kindt T J
J Immunol. 1980 Jun;124(6):2864-9.
CH allotypes were determined for rabbit IgG and antibody molecules identified and isolated on the basis of their expression of latent group a (VH) allotypic determinants. Serologic and chemical typing of the purified molecules revealed that five out of 10 individual preparations studied expressed d allotypes not present in the rabbit as a nominal allotype. None of the latent VH-CH combinations represented products of a new or unusual arrangement of VH and CH structural genes. This result indicates that the molecules with latent allotypes express their VH and CH allotypes in the same combinations as do their nominal counterparts and do not result from misspairing of separate genes involved in H chain synthesis.
测定了兔IgG的CH同种异型，并根据潜在的a组（VH）同种异型决定簇的表达对抗体分子进行了鉴定和分离。对纯化分子的血清学和化学分型显示，在所研究的10个个体制剂中，有5个表达了兔作为名义同种异型不存在的d同种异型。没有一个潜在的VH-CH组合代表VH和CH结构基因新的或异常排列的产物。这一结果表明，具有潜在同种异型的分子以与其名义对应物相同的组合表达其VH和CH同种异型，并非由参与重链合成的单独基因的错配所致。
