兔IgG重链基因：编码γ重链的cDNA的分离及两个基因组Cγ基因的鉴定

Heavy chain genes of rabbit IgG: isolation of a cDNA encoding gamma heavy chain and identification of two genomic C gamma genes.

作者信息

Martens C L, Moore K W, Steinmetz M, Hood L, Knight K L

出版信息

Proc Natl Acad Sci U S A. 1982 Oct;79(19):6018-22. doi: 10.1073/pnas.79.19.6018.

DOI:10.1073/pnas.79.19.6018

PMID:6193512

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC347043/

Abstract

A cDNA library was constructed by using rabbit spleen poly(A)+RNA as template, and from this library was isolated a cDNA clone, p2a2, that encodes 179 amino acids of the heavy chain of rabbit IgG. The nucleotide sequence of p2a2 showed that it encodes the COOH-terminal eight amino acids of the CH1 domain, the hinge region, the CH2 domain, and the NH2-terminal half of the CH3 domain of C gamma. Southern blot hybridization analysis of rabbit sperm DNA showed that two EcoRI fragments hybridized strongly with the C gamma cDNA. The p2a2 cDNA was used as a probe to isolate recombinant Charon 4A phage clones containing C gamma sequences from a genomic library of rabbit liver DNA. Two distinct DNA segments were identified by restriction mapping and hybridization analysis, suggesting that the haploid rabbit genome may contain two different C gamma genes.

摘要

以兔脾脏多聚腺苷酸（poly(A)）+RNA为模板构建了一个cDNA文库，从该文库中分离出一个cDNA克隆p2a2，它编码兔IgG重链的179个氨基酸。p2a2的核苷酸序列表明，它编码Cγ的CH1结构域的COOH末端八个氨基酸、铰链区、CH2结构域以及CH3结构域的NH2末端一半。兔精子DNA的Southern印迹杂交分析表明，两个EcoRI片段与Cγ cDNA强烈杂交。p2a2 cDNA用作探针，从兔肝脏DNA基因组文库中分离出含有Cγ序列的重组Charon 4A噬菌体克隆。通过限制性图谱分析和杂交分析鉴定出两个不同的DNA片段，这表明单倍体兔基因组可能包含两个不同的Cγ基因。