Application of high-pressure liquid chromatography and thermal energy analyzer to analysis of trinitroglycerin and its metabolites in blood.

A highly selective and sensitive analytical procedure for the determination of trinitroglycerin and four metabolites in whole blood was developed. Trinitroglycerin and its metabolites were extracted from whole blood with ethyl acetate and analyzed by high-pressure liquid chromatography using the thermal energy analyzer detector. Linearity of response was observed over the 1-1000-ng range. The applicability of this method to the analysis of whole blood from dogs orally dosed with trinitroglycerin is described.