Baba S, Shinohara Y, Sano H, Inoue T, Masuda S, Kurono M
J Chromatogr. 1984 Jan 13;305(1):119-26. doi: 10.1016/s0378-4347(00)83319-8.
A new, sensitive, and specific high-performance liquid chromatographic method for the quantitative analysis of [14C]glyceryl trinitrate and its four metabolites in plasma is described. The drugs are extracted from 0.05 ml of plasma with methanol and analyzed by high-performance liquid chromatography using a synchronized accumulating radioisotope detector. The limit of detection is 0.2 ng per injection. The within-day coefficient of variation is 5.9% at a concentration of 27.0 ng per ml of plasma. The method was applied to single-dose pharmacokinetics of glyceryl trinitrate in rat.
本文描述了一种用于定量分析血浆中[¹⁴C]甘油三硝酸酯及其四种代谢物的新型、灵敏且特异的高效液相色谱法。药物从0.05 ml血浆中用甲醇萃取，并使用同步累积放射性同位素检测器通过高效液相色谱法进行分析。检测限为每次注射0.2 ng。在血浆浓度为每毫升27.0 ng时，日内变异系数为5.9%。该方法应用于大鼠体内甘油三硝酸酯的单剂量药代动力学研究。
