Le Menn F, Rochefort H, Garcia M
Steroids. 1980 Mar;35(3):315-28. doi: 10.1016/0039-128x(80)90044-6.
We have investigated the action of high doses of androgens in Gobius niger L., a marine teleostean fish, by characterizing specific steroid receptors in liver and by assaying the plasma vitellogenin concentration under different hormonal treatments. Estrogen and androgen receptors were characterized in the liver nuclear extracts according to their binding specificity. The maximum binding capacity was 25 fmoles/mg protein for the estrogen and androgen receptors. In vivo, high doses of DHT()increased the concentration of plasmatic vitellogenin as assayed by immunodiffusion while low doses were inefficient. In spite of a similar number of estrogen and androgen nuclear receptor sites (25 fmoles/mg protein), DHT was at least 70 fold less active than E2 on yolk protein and vitellogenin induction both in male and female Gobius niger. In addition, the antiestrogen tamoxifen, which was inactive by itself, inhibited the E2 and the DHT induced accumulation of vitellogenin. Progesterone (2 mg/fish) was also totally inactive in inducing vitellogenin. We conclude that the induction of vitellogenin by DHT is mediated by the estrogen receptor rather than by the androgen receptor. In addition to the estradiol induced protein in rat uterus and to other estrogenic responses obtained by androgens in mammary cancer, fish vitellogenin is another estrogen regulated protein which can be induced by high doses of androgens.
我们通过鉴定肝脏中的特异性类固醇受体以及测定不同激素处理下血浆卵黄蛋白原浓度，研究了高剂量雄激素对黑腹美鰕虎鱼（一种海洋硬骨鱼）的作用。根据雌激素和雄激素受体的结合特异性，对肝脏核提取物中的受体进行了鉴定。雌激素和雄激素受体的最大结合容量为25飞摩尔/毫克蛋白。在体内，通过免疫扩散测定，高剂量的双氢睾酮（DHT）可增加血浆卵黄蛋白原的浓度，而低剂量则无效。尽管雌激素和雄激素核受体位点数量相似（25飞摩尔/毫克蛋白），但在雄性和雌性黑腹美鰕虎鱼中，DHT在诱导卵黄蛋白和卵黄蛋白原方面的活性至少比雌二醇（E2）低70倍。此外，本身无活性的抗雌激素他莫昔芬可抑制E2和DHT诱导的卵黄蛋白原积累。孕酮（2毫克/鱼）在诱导卵黄蛋白原方面也完全无活性。我们得出结论，DHT诱导卵黄蛋白原是由雌激素受体介导的，而非雄激素受体。除了大鼠子宫中雌二醇诱导的蛋白质以及雄激素在乳腺癌中获得的其他雌激素反应外，鱼类卵黄蛋白原是另一种可由高剂量雄激素诱导的雌激素调节蛋白。
