Gschwendt M, Rincke G, Schuster T
Mol Cell Endocrinol. 1982 Apr;26(1-2):231-42. doi: 10.1016/0303-7207(82)90020-x.
(1) The induction of vitellogenin synthesis in chicken liver by an estrogen implant as well as the decline of vitellogenin synthesis and nuclear receptor concentration after withdrawal of the implant were studied. For the detection of vitellogenin the SAC immunoprecipitation technique was used. The nuclear receptor decreases very rapidly and somewhat earlier than the capacity of the liver to synthesize vitellogenin. (2) The inhibition by antiestrogens of the estrogen-induced vitellogenin synthesis as well as of the accumulation of the estrogen-receptor complex in the nucleus was investigated. Tamoxifen as well as the recently described 1,1,2,2-tetramethyl-1,2-bis(4'-hydroxyphenyl)ethane were found to be true antiestrogens in the chicken liver, i.e. they inhibit estrogen-induced vitellogenin synthesis but themselves cause no induction.
(1) 研究了雌激素植入物对鸡肝脏中卵黄蛋白原合成的诱导作用，以及植入物取出后卵黄蛋白原合成和核受体浓度的下降情况。采用SAC免疫沉淀技术检测卵黄蛋白原。核受体下降非常迅速，且比肝脏合成卵黄蛋白原的能力下降稍早。(2) 研究了抗雌激素对雌激素诱导的卵黄蛋白原合成以及雌激素受体复合物在细胞核中积累的抑制作用。发现他莫昔芬以及最近描述的1,1,2,2-四甲基-1,2-双(4'-羟基苯基)乙烷在鸡肝脏中是真正的抗雌激素，即它们抑制雌激素诱导的卵黄蛋白原合成，但自身不会引起诱导。
