Ruedas G
Urol Int. 1980;35(2):81-90. doi: 10.1159/000280308.
Dog kidneys have been cold perfused by gravity and by nonpulsatile pressure after 24 h preservation time at 5 degrees C with normotonic Ringer's solution without drugs, Ringer's solution containing furosemide (10 mg/l), acetylcholine (100 mg/l), phentolamine (10 mg/l) or dopamine (0.2, 2 and 20 mg/l), and Ringer's solution made hypertonic (400 and 500 mosm/l) with glucose, mannitol, choline chloride or urea. Addition of the described drugs to normotonic Ringer's produced no detectable change in the perfusion rate. In contrast, a significant flow increase was constantly observed when perfusion was made with hypertonic Ringer's solutions. Flow increase was found to be dependent on the osmolarity of the perfusate but not on the kind of solute used to raise it.
在5℃下保存24小时后，用无药物的等渗林格氏液、含呋塞米（10毫克/升）、乙酰胆碱（100毫克/升）、酚妥拉明（10毫克/升）或多巴胺（0.2、2和20毫克/升）的林格氏液，以及用葡萄糖、甘露醇、氯化胆碱或尿素制成高渗（400和500毫渗摩尔/升）的林格氏液，通过重力和非搏动压力对犬肾进行冷灌注。向等渗林格氏液中添加所述药物后，灌注速率未出现可检测到的变化。相比之下，当用高渗林格氏液进行灌注时，始终观察到显著的流量增加。发现流量增加取决于灌注液的渗透压，而不取决于用于提高渗透压的溶质种类。
