Peschek G A
Biochem J. 1980 Feb 15;186(2):515-23. doi: 10.1042/bj1860515.
Electron transport from H2, NADPH, NADH and succinate to O2 or ferricytochrome c in respiratory particles isolated from Anacystis nidulans in which hydrogenase had been induced was abolished after extraction of the membranes with n-pentane; oxidation of ascorbate plus NNN'N'-tetramethyl-p-phenylenediamine remained unaffected. Incorporation of authentic ubiquinone-10, plastoquinone-9, menaquinone-7 and phylloquinone (in order of increasing efficiency) restored the electron-transport reactions. ATP-dependent reversed electron flow from NNN'N'-tetramethyl-p-phenylenediamine to NADP+ or, via the membrane-bound hydrogenase, to H+ was likewise abolished by pentane extraction and restored by incorporation of phylloquinone. Participation of the incorporated quinones in the respiratory electron-transport reactions of reconstituted particles was confirmed by measuring the degree of steady-state reduction of the quinones. Isolation and identification of the quinones present in native Anacystis membranes yielded mainly plastoquinone-9 and phylloquinone; neither menaquinone nor alpha-tocopherolquinone could be detected. Together with the results from reconstitution experiments this suggests that phylloquinone might function as the main respiratory quinone in Anacystis nidulans.
在用正戊烷提取膜后，从已诱导氢化酶的集胞藻中分离出的呼吸颗粒中，从H₂、NADPH、NADH和琥珀酸到O₂或铁细胞色素c的电子传递被消除；抗坏血酸加NNN'N'-四甲基对苯二胺的氧化不受影响。加入 authentic 泛醌-10、质体醌-9、甲基萘醌-7和叶绿醌（按效率递增顺序）可恢复电子传递反应。从NNN'N'-四甲基对苯二胺到NADP⁺或通过膜结合氢化酶到H⁺的ATP依赖性逆向电子流同样因戊烷提取而被消除，并通过加入叶绿醌而恢复。通过测量醌的稳态还原程度，证实了掺入的醌参与了重组颗粒的呼吸电子传递反应。对集胞藻天然膜中存在的醌进行分离和鉴定，主要得到质体醌-9和叶绿醌；未检测到甲基萘醌和α-生育酚醌。结合重组实验的结果，这表明叶绿醌可能是集胞藻中的主要呼吸醌。
