蓝藻中是否含有“哺乳动物型”细胞色素氧化酶？

Do cyanobacteria contain "mammalian-type" cytochrome oxidase?

作者信息

Peschek G A, Schmetterer G, Lauritsch G, Nitschmann W H, Kienzl P F, Muchl R

出版信息

Arch Microbiol. 1982 May;131(3):261-5. doi: 10.1007/BF00405890.

Abstract

The cytochrome content of membranes isolated from seven species of cyanobacteria was investigated in terms of conventional difference spectra, carbon monoxide difference spectra, photoaction spectra and photodissociation spectra, and by extraction of acid-labile heme followed by spectral identification. In addition, the effect of various inhibitors and activators on the oxidation of horse heart cytochrome c by the membrane was studied. Both the spectral features and the properties of the cytochrome oxidase reaction catalysed by the membranes suggested the presence of a terminal oxidase strikingly similar to mitochondrial ferrocytochrome c:oxygen oxidoreductase (EC. 1.9.3.1).

摘要

从七种蓝细菌中分离出的膜的细胞色素含量，通过常规差光谱、一氧化碳差光谱、光作用光谱和光解离光谱，并通过酸不稳定血红素的提取及随后的光谱鉴定进行了研究。此外，还研究了各种抑制剂和激活剂对膜催化马心细胞色素c氧化的影响。膜的光谱特征和细胞色素氧化酶反应的性质均表明存在一种与线粒体亚铁细胞色素c：氧氧化还原酶（EC. 1.9.3.1）极为相似的末端氧化酶。