[能够翻译终止密码子U-G-A的酵母线粒体色氨酸-tRNA的一级结构]

[Primary structure of yeast mitochondrial tryptophan-tRNA capable of translating the termination U-G-A codon].

作者信息

Sibler A P, Bordonné R, Dirheimer G, Martin R

出版信息

C R Seances Acad Sci D. 1980 Mar 17;290(11):695-8.

Abstract

The primary structure of Yeast Saccharomyces cerevisiae mitochondrial tryptophane-tRNA, isolated by reversed phase chromatography and two-dimensional gel electrophoresis has been determined. It is as follows: pA-A-G-G-A-U-A-U-A-G-U-U-U-A-A-D-G-G-D-A-A-A-A-C-A-G-U-U-G-A-psi-U-U-C-A-i6 A (ms2i6 A)-A-psi-C-A-A-U-C-A-U-U-A-G-G-A-G-T-psi-C-G-A-A-U-C-U-C-U-U-U-A-U-C-C-U-U-G-C-C-A. Owing to its anticodon U-C-A-, where U represents a modified uridine, this tRNA can translate the opal codon U-G-A, which is usually a protein synthesis termination codon in cytoplasmic systems.

摘要

通过反相色谱法和二维凝胶电泳分离得到的酿酒酵母线粒体色氨酸 - tRNA的一级结构已被确定。其序列如下：pA - A - G - G - A - U - A - U - A - G - U - U - U - A - A - D - G - G - D - A - A - A - A - C - A - G - U - U - G - A - ψ - U - U - C - A - i6A（ms2i6A） - A - ψ - C - A - A - U - C - A - U - U - A - G - G - A - G - T - ψ - C - G - A - A - U - C - U - C - U - U - U - A - U - C - C - U - U - G - C - C - A。由于其反密码子为U - C - A - ，其中U代表一种修饰的尿苷，这种tRNA能够翻译通常在细胞质系统中作为蛋白质合成终止密码子的乳白密码子U - G - A。