胎猴骨骼的三色荧光活体标记
Trichromatic fluorescent vital labeling of bone in the fetal macque.
作者信息
Newell-Morris L, Tarrant L H, Sirianni J E, Munger R G
出版信息
Experientia. 1980 May 15;36(5):623-4. doi: 10.1007/BF01965841.
PMID:6769705
Abstract
Mineralizing tissue was labeled in the macaque fetus by administering sequential vital bone labels to the pregnant female. The 3 labels used (DCAF, xylenol orange and minocycline) fluoresce different colors, thereby facilitating identification of discrete lines in the rapidly growing bone and a quantitative analysis of bone deposition in utero.
摘要
通过给怀孕的雌性猕猴连续施用活体骨标记物，在猕猴胎儿中标记矿化组织。所使用的三种标记物（二氯荧光素、二甲酚橙和米诺环素）发出不同颜色的荧光，从而便于识别快速生长骨骼中的离散线条，并对子宫内的骨沉积进行定量分析。
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