Sun T C, Mori S, Roper J, Brown C, Hooser T, Burr D B
Department of Anatomy, Indiana University, Indianapolis 46202.
Bone. 1992;13(6):443-6. doi: 10.1016/8756-3282(92)90088-e.
Substances that bind calcium are given to determine where and how fast bone is forming. Several vital dyes are used (tetracycline, calcein, alizarin, xylenol), but it is not known whether the histomorphometric results they provide are equivalent. This work tests whether different fluorochrome labels give the same results when they are quantitatively measured. Twelve-week-old rats (n = 58) were divided into six groups and given double labels IP of calcein, tetracycline HCl, alizarin complexone, or xylenol using a 1-7-1 scheme. Two other groups received either calcein followed by tetracycline, or tetracycline followed by calcein. Our results show that (a) tetracycline hydrochloride leads to a significant underestimation of mineralizing surface when given as the second label, probably because of its weak fluorescence; (b) there were no differences among any of the non-tetracycline labels for any of the histomorphometric measurements; (c) there was no evidence of osteoblast suppression with any label; and (d) there was no evidence that tetracycline caused increased osteoblast resting periods.
给予能结合钙的物质以确定骨骼形成的位置和速度。使用了几种活性染料（四环素、钙黄绿素、茜素、二甲苯酚），但尚不清楚它们所提供的组织形态计量学结果是否等效。这项工作测试了不同的荧光染料标记在进行定量测量时是否给出相同的结果。将12周龄的大鼠（n = 58）分为六组，按照1-7-1方案经腹腔注射给予钙黄绿素、盐酸四环素、茜素络合物或二甲苯酚的双重标记。另外两组分别先给予钙黄绿素后给予四环素，或先给予四环素后给予钙黄绿素。我们的结果表明：（a）当作为第二个标记给予时，盐酸四环素会导致矿化表面的显著低估，可能是因为其荧光较弱；（b）对于任何组织形态计量学测量，任何非四环素标记之间均无差异；（c）没有证据表明任何标记会抑制成骨细胞；（d）没有证据表明四环素会导致成骨细胞静止期增加。
