Mott G E, Jackson E M
J Lipid Res. 1980 May;21(4):480-4.
After oral administration of a mixture of [1,2(n)-3H]cholesterol and [4-14C]cholesterol to a baboon, fecal coprostanone had a 46% lower 3H/14C ratio than the dose administered. Loss of 3H by enolization of the 3-ketone could account for the decrease in 3H/14C. If [7(n)-3H]cholesterol was administered instead of [1,2(n)-3H]cholesterol a 23% loss of 3H from coprostanone was found. Procedures requiring measurement of 3H-coprostanone derived from [1,2(n)-3H]- or [7(n)-3H]cholesterol could be seriously in error unless an appropriate correction for loss of 3H is made.
给一只狒狒口服[1,2(n)-3H]胆固醇和[4-14C]胆固醇的混合物后，粪便中的粪甾烷酮的3H/14C比值比给药剂量低46%。3-酮的烯醇化导致3H的损失可以解释3H/14C的降低。如果给予的是[7(n)-3H]胆固醇而不是[1,2(n)-3H]胆固醇，则发现粪甾烷酮中有23%的3H损失。除非对3H的损失进行适当校正，否则需要测量源自[1,2(n)-3H]-或[7(n)-3H]胆固醇的3H-粪甾烷酮的程序可能会出现严重误差。
