Wren M W
J Med Microbiol. 1980 May;13(2):257-63. doi: 10.1099/00222615-13-2-257.
The value of prolonged primary anaerobic incubation for the isolation of anaerobic bacteria from clinical material was assessed by comparison of two incubation systems. Continuous incubation of primary anaerobic plates for 48 h was superior to conventional methods in that more anaerobes were isolated. The results indicate that in some cases prolonged incubation may be the only method of achieving the correct bacteriological diagnosis, particularly when relatively oxygen-sensitive strains are involved or when organisms may be damaged by exposure to oxygen during transport. Use of extended primary incubation provides a clearer picture of the microbial flora of the original lesion and reveals that some anaerobic genera may be more commonly involved than is at present recognised.
通过比较两种培养系统，评估了延长初次厌氧培养时间对从临床材料中分离厌氧菌的价值。初次厌氧平板连续培养48小时比传统方法更具优势，因为分离出的厌氧菌更多。结果表明，在某些情况下，延长培养时间可能是获得正确细菌学诊断的唯一方法，特别是当涉及相对氧敏感菌株或当生物体在运输过程中可能因暴露于氧气而受损时。使用延长的初次培养能更清楚地了解原始病变的微生物群落，并揭示一些厌氧菌属可能比目前公认的更常见。
