Bresler S E, Kalinin V L, Kreneva R A
Mol Gen Genet. 1980;177(4):691-8. doi: 10.1007/BF00272681.
The relative yield (Nm/N) of fluorescent mutants Ind- after the transformation of Bacillus subtilis cells by means of UV-irradiated DNA is much higher in an uvr- recipient than in an uvr+ strain, when compared at equal fluence, but practically identical at equal survival. Ind- mutations are induced by UV-irradiation of separated single strands of transforming DNA. The H-strand is much more sensitive to the mutagenic action of UV light. Preliminary irradiation of competent recipient cells by moderate UV fluences increases the survival of UV- or gamma-irradiated transforming DNA (W-reactivation) and the frequency of Ind- mutations (W-mutagenesis). During transfection of B. subtilis cells by UV-irradiated prophage DNA isolated from lysogenic cells B. subtilis (phi 105 c+) c-mutants of the phage are obtained in high yield only in conditions of W-mutagenesis, i.e. in UV-irradiated recipient cells. These data show that there is no substantial spontaneous induction of error-prone SOS-repair system in the competent cells of B. subtilis.
当以相等通量进行比较时，用紫外线照射的DNA转化枯草芽孢杆菌细胞后，荧光突变体Ind-的相对产量（Nm/N）在uvr-受体中比在uvr+菌株中高得多，但在相等存活率时实际上是相同的。Ind-突变是由转化DNA的单链经紫外线照射诱导产生的。H链对紫外线的诱变作用更为敏感。用适度的紫外线通量预先照射感受态受体细胞，可提高紫外线或γ射线照射的转化DNA的存活率（W复活）以及Ind-突变的频率（W诱变）。在用从溶源性细胞枯草芽孢杆菌（phi 105 c+）分离的紫外线照射的原噬菌体DNA转染枯草芽孢杆菌细胞期间，仅在W诱变条件下，即在紫外线照射的受体细胞中，才能高产率地获得噬菌体的c-突变体。这些数据表明，在枯草芽孢杆菌的感受态细胞中不存在明显的易出错SOS修复系统的自发诱导。
