Rutberg L, Hoch J A, Spizizen J
J Virol. 1969 Jul;4(1):50-7. doi: 10.1128/JVI.4.1.50-57.1969.
Bacteriophage phi105 is a temperate phage for the transformable Bacillus subtilis 168. The infectivity of deoxyribonucleic acid (DNA) extracted from mature phi105 phage particles, from bacteria lysogenic for phi105 (prophage DNA), and from induced lysogenic bacteria (vegetative DNA) was examined in the B. subtilis transformation system. About one infectious center was formed per 10(8) mature DNA molecules added to competent cells, but single markers could be rescued from mature DNA by a superinfecting phage at a 10(3)- to 10(4)-fold higher frequency. Single markers in mature DNA were inactivated at an exponential rate after uptake by a competent cell. Prophage and vegetative DNA gave about one infectious center per 10(3) molecules added to competent cells. Infectious prophage DNA entered competent cells as a single molecule; it gave a majority of lytic responses. Single markers in sheared prophage DNA were inactivated at the same rate as markers in mature DNA. Prophage DNA was dependent on the bacterial rec-1 function for its infectivity, whereas vegetative DNA was not. The mechanism of transfection of B. subtilis with viral DNA is discussed, and a model for transfection with phi105 DNA is proposed.
噬菌体phi105是可转化的枯草芽孢杆菌168的温和噬菌体。在枯草芽孢杆菌转化系统中检测了从成熟的phi105噬菌体颗粒、phi105溶原性细菌（原噬菌体DNA）以及诱导的溶原性细菌（营养DNA）中提取的脱氧核糖核酸（DNA）的感染性。向感受态细胞中添加每10^8个成熟DNA分子大约形成一个感染中心，但通过超感染噬菌体从成熟DNA中拯救单个标记的频率要高10^3到10^4倍。成熟DNA中的单个标记在被感受态细胞摄取后以指数速率失活。向感受态细胞中添加每10^3个原噬菌体和营养DNA分子大约产生一个感染中心。感染性原噬菌体DNA作为单个分子进入感受态细胞；它产生大多数裂解反应。剪切后的原噬菌体DNA中的单个标记与成熟DNA中的标记以相同速率失活。原噬菌体DNA的感染性依赖于细菌的rec-1功能，而营养DNA则不依赖。讨论了用病毒DNA转染枯草芽孢杆菌的机制，并提出了用phi105 DNA转染的模型。
