Friedman Z, Demers L M
Pediatr Res. 1980 Mar;14(3):190-3. doi: 10.1203/00006450-198003000-00003.
Prostaglandins play an important role in renal physiology. We have studied the prostaglandin synthetase system in 10 human newborn kidneys ranging from 22 to 40 weeks of gestation who succumbed within 40 hr postnatally. Slices of medulla and cortex were incubated within 2 hr of death in a medium containing 50 microgram arachidonic acid and 50 muCi [3H]arachidonic acid. The incoroporation of [3H]AA into prostaglandin (PG)E and PGF was determined at 30-min intervals in medulla and cortex tissue and in the incubation media over 120 min and at 180 min, using silicic acid chromatography and scintillation counting. The study showed that biosynthesis of the primary prostaglandins occurs mainly in the renal medulla as early as 22 weeks of gestation, with the biosynthetic activity increasing concomitantly with advanced gestation. The ratio of PGE to PGF in the medulla and cortex was always greater than 1. The ratio was obtained in kidneys from neonates born before 28 weeks of gestation. Because prostaglandins are biosynthesized in the neonatal kidney, it is conceivable that hemodynamic and fluid and electrolyte homeostasis functions of prostaglandins may exist at this very early stage of development.
前列腺素在肾脏生理学中发挥着重要作用。我们研究了10例妊娠22至40周的人类新生儿肾脏中的前列腺素合成酶系统，这些新生儿在出生后40小时内死亡。在死亡后2小时内，将髓质和皮质切片置于含有50微克花生四烯酸和50微居里[3H]花生四烯酸的培养基中孵育。使用硅酸色谱法和闪烁计数法，在120分钟内每隔30分钟以及在180分钟时，测定髓质和皮质组织以及孵育培养基中[3H]花生四烯酸掺入前列腺素（PG）E和PGF的情况。研究表明，早在妊娠22周时，主要前列腺素的生物合成主要发生在肾髓质中，且生物合成活性随着妊娠进展而增加。髓质和皮质中PGE与PGF的比值始终大于1。该比值是在妊娠28周前出生的新生儿的肾脏中获得的。由于前列腺素在新生儿肾脏中生物合成，因此可以设想，前列腺素的血流动力学以及液体和电解质稳态功能可能在发育的这个非常早期阶段就已存在。
