Pong S S, Levine L
Res Commun Chem Pathol Pharmacol. 1976 Jan;13(1):115-23.
Prostaglandin biosynthetic activity has been found in the microsomal fractions prepared from rabbit renal cortex. The cortex preparation requires arachidonic acid as substrate and hydroquinone and reduced glutathione as cofactors for enzymatic activity and is inhibited by indomethacin. The biosynthetic capacity of the washed microsomes of cortex ranges from one third to equal that of the washed microsomes of medulla. A partially purified medullary cytoplasmic factor and crystalline hemoglobin can stimulate greatly the synthetase activity in the microsomes of the medulla, but they have no effect on the activity of cortical microsomes.
已在从兔肾皮质制备的微粒体组分中发现前列腺素生物合成活性。皮质制剂需要花生四烯酸作为底物，对苯二酚和还原型谷胱甘肽作为酶活性的辅助因子，并且受到吲哚美辛的抑制。皮质洗涤微粒体的生物合成能力为髓质洗涤微粒体的三分之一至相同水平。部分纯化的髓质细胞质因子和结晶血红蛋白可极大地刺激髓质微粒体中的合成酶活性，但它们对皮质微粒体的活性没有影响。
