Eastwood G L, Erdmann K R
Am J Dig Dis. 1978 May;23(5):429-35. doi: 10.1007/BF01072926.
We correlated changes in the gastric transmucosal potential difference (PD), as an indicator of the integrity of the gastric mucosal barrier, with morphological evidence of injury in dogs which had received either intragastric saline or 5, 10, 15, or 30% ethanol. Increasing degrees of morphological damage were accompanied by greater, more rapid changes in PD. Furthermore, ultrastructural changes occurred within surface epithelial cells, not in the deeper parietal or zymogen cells, and initially did not involve disruption of the apical cell membrane. Typically, the tight junctions also were not affected, although in a minority of dogs small bleblike separations of the tight junctions were seen. We consider the gastric mucosal barrier to be represented morphologically by the interconnecting sheet of gastric epithelial cells and that ethanol breaks the barrier by first causing intracellular injury.
我们将胃黏膜跨膜电位差（PD）的变化（作为胃黏膜屏障完整性的指标）与接受胃内生理盐水或5%、10%、15%或30%乙醇的犬类损伤的形态学证据进行了关联。形态学损伤程度的增加伴随着PD更大、更快的变化。此外，超微结构变化发生在表面上皮细胞内，而非更深层的壁细胞或酶原细胞内，并且最初并不涉及顶端细胞膜的破坏。通常，紧密连接也未受影响，尽管在少数犬类中可见紧密连接有小泡状分离。我们认为胃黏膜屏障在形态学上由相互连接的胃上皮细胞片层代表，并且乙醇首先通过引起细胞内损伤来破坏屏障。
