Lopes-Virella M F, Virella G, Evans G, Malenkos S B, Colwell J A
Clin Chem. 1980 Jul;26(8):1205-8.
We report an immunonephelometric assay for apolipoprotein AI, which involves use of a laser nephelometer and an antiserum prepared in our laboratory. Coefficients of variation were 2.5% within-run, 6.3% between-run. Several types of manipulation of the samples (heating, delipidation, dissociation with urea), reportedly necessary for accurate determinations by radioimmunoassay or gel diffusion immunoassay, did not substantially affect results obtained with our technique. The assay is sensitive, reproducible, and simple, and appears suitable for routine use.
我们报告了一种载脂蛋白AI的免疫比浊测定法，该方法使用了激光比浊仪和我们实验室制备的抗血清。批内变异系数为2.5%，批间变异系数为6.3%。据报道，放射免疫测定法或凝胶扩散免疫测定法为进行准确测定所需的几种样品处理方式（加热、脱脂、用尿素解离），对我们这项技术所获得的结果没有实质性影响。该测定法灵敏、可重复且操作简单，似乎适用于常规使用。
