A simplified technique of high resolution two-dimensional electrophoresis: serum immunoglobulins.

A relatively simple, inexpensive technique is described for high resolution two-dimensional electrophoresis of human serum proteins. The technique clearly resolves the heavy chains of IgG, IgA and IgM, the positions of which have now been established. The technique is highly reproducible for normal serum. Distinct changes were observed upon examination of the sera of patients with IgG OF IgA myelomatosis or Waldenström's macroglobulinaemia. The technique should prove valuable in both the study of immunoglobulin disorders and studies into the fine structure of highly purified immunoglobulins.