Latner A L, Marshall T, Gambie M
Clin Chim Acta. 1980 Apr 11;103(1):51-9. doi: 10.1016/0009-8981(80)90230-2.
A relatively simple, inexpensive technique is described for high resolution two-dimensional electrophoresis of human serum proteins. The technique clearly resolves the heavy chains of IgG, IgA and IgM, the positions of which have now been established. The technique is highly reproducible for normal serum. Distinct changes were observed upon examination of the sera of patients with IgG OF IgA myelomatosis or Waldenström's macroglobulinaemia. The technique should prove valuable in both the study of immunoglobulin disorders and studies into the fine structure of highly purified immunoglobulins.
本文描述了一种相对简单、成本低廉的技术，用于人血清蛋白的高分辨率二维电泳。该技术能够清晰地分离出IgG、IgA和IgM的重链，其位置现已确定。对于正常血清，该技术具有高度的可重复性。在检查IgG或IgA骨髓瘤患者以及瓦尔登斯特伦巨球蛋白血症患者的血清时，观察到了明显的变化。该技术在免疫球蛋白疾病的研究以及高纯度免疫球蛋白精细结构的研究中都应具有重要价值。
