Westerbrink K, Warthold U, Hebbeln J, Postel E, Havsteen B
J Cancer Res Clin Oncol. 1981;101(1):43-51. doi: 10.1007/BF00405062.
The technique of high-resolution, two-dimensional electrophoresis was applied to crude membrane protein fractions of various normal human tissue samples, and those, mainly spleens and lymph nodes, of patients with lymphomas of different types. The obtained protein maps show a high reproducibility, tissue specificity, and sensitivity to specific oncologic changes. The relevance of the latter aspect to tumor characterization is illustrated. Quantitatively, minor proteins were detected by a sensitive silver stain. The feasibility of objective, computerized quantification and evaluation of the protein maps is described.
高分辨率二维电泳技术被应用于各种正常人体组织样本以及不同类型淋巴瘤患者的粗膜蛋白组分，这些患者的样本主要来自脾脏和淋巴结。所获得的蛋白质图谱显示出高重现性、组织特异性以及对特定肿瘤变化的敏感性。文中阐述了后一方面与肿瘤特征描述的相关性。在定量方面，通过灵敏的银染检测到微量蛋白质。本文描述了对蛋白质图谱进行客观、计算机化定量和评估的可行性。
