Muto N, Murayama K, Akahane K, Tani S
J Biochem. 1980 Mar;87(3):717-23. doi: 10.1093/oxfordjournals.jbchem.a132800.
Biochemical and immunological properties of two kinds of pepsinogens isolated from the gastric mucosal extracts of adult Wistar rats were studied. Their activated enzymes were prepared from the zymogens using a DEAE-Sepharose CL-6B column. The isoelectric points of pepsinogens I and II were estimated to be 3.90 and 3.75, respectively, by isoelectric focusing, and those of pepsins I and II to be 3.60 and 3.45, respectively. Amino acid compositions of the two pepsinogens or pepsins were strikingly similar to each other and neither pepsinogen I nor II contained organic phosphate. The biochemical properties of rat preparations compared with porcine pepsinogens A and C and pepsins A [EC 3.4.23.1] and C [EC 3.4.23.3] showed that rat pepsinogens and pepsins resembled porcine pepsinogen C and pepsin C, respectively. Pepsinogens I and II were demonstrated to share a similar immunogenic molecular structure by double diffusion analysis and Laurell immunoelectrophoresis. Rabbit antipepsinogen I serum cross-reacted with the mouse preparation but did not with the rabbit and porcine preparations. The possibility of the genetically controlled occurrence of pepsinogens I and II in the rat is discussed.
对从成年Wistar大鼠胃黏膜提取物中分离出的两种胃蛋白酶原的生化和免疫学特性进行了研究。使用DEAE-琼脂糖CL-6B柱从酶原制备它们的活化酶。通过等电聚焦法估计胃蛋白酶原I和II的等电点分别为3.90和3.75，胃蛋白酶I和II的等电点分别为3.60和3.45。两种胃蛋白酶原或胃蛋白酶的氨基酸组成彼此极为相似，且胃蛋白酶原I和II均不含有机磷酸盐。将大鼠制品的生化特性与猪胃蛋白酶原A和C以及胃蛋白酶A [EC 3.4.23.1]和C [EC 3.4.23.3]进行比较，结果表明大鼠胃蛋白酶原和胃蛋白酶分别类似于猪胃蛋白酶原C和胃蛋白酶C。通过双向扩散分析和劳雷尔免疫电泳证明胃蛋白酶原I和II具有相似的免疫原性分子结构。兔抗胃蛋白酶原I血清与小鼠制品发生交叉反应，但与兔和猪制品不发生交叉反应。讨论了大鼠中胃蛋白酶原I和II受基因控制出现的可能性。
