Palit A, Harrison P M
J Gen Microbiol. 1980 Mar;117(1):141-6. doi: 10.1099/00221287-117-1-141.
Antigens of the outer envelope from Leptospira interrogans serovar canicola (Hond Utrecht IV) were extracted by 50% (v/v) ethanol or by sodium dodecyl sulphate and serological analysis suggested that they were identical. The "fraction 4" extracted by alkali was found to contain glycoproteins of high (retentate) and low (filtrate) molecular weight; the latter behaved like a hapten in serology and in animal immunization experiments. Antibodies were raised in rabbits against this hapten by conjugating it to bovine albumin fraction V. The antiserum was found to react with both the low molecular weight and high molecular weight glycoproteins. This anti-hapten serum contained little or no whole-cell-agglutinating antibodies. The fraction 4 retentate behaved like a complete antigen in serological and immunization studies. Fraction 4 retentate and the outer envelope preparations were serologically related but they were not identical. Chemical studies revealed similarities between the carbohydrate component of the outer envelope obtained by ethanol extraction and fraction 4. The outer envelope extracted by ethanol, fraction 4 and its low and high molecular weight glycoproteins contained arabinose, rhamnose, fucose, xylose, mannose, galactose, glucose, glucosamine and glucuronic acid. Three unidentified peaks were observed in gas-liquid chromatographic analysis of the O-trimethylsilyl derivatives of methyl glycosides of all these samples and one of these peaks co-eluted with the O-trimethylsilyl derivative of 3-O-methylmannose.
问号钩端螺旋体犬血清型（洪都拉斯乌得勒支IV型）外膜抗原用50%（v/v）乙醇或十二烷基硫酸钠提取，血清学分析表明它们是相同的。发现用碱提取的“组分4”含有高分子量（截留物）和低分子量（滤液）的糖蛋白；后者在血清学和动物免疫实验中表现得像一种半抗原。通过将这种半抗原与牛血清白蛋白V组分偶联，在兔体内产生了针对该半抗原的抗体。发现抗血清与低分子量和高分子量糖蛋白都有反应。这种抗半抗原血清几乎没有或没有全细胞凝集抗体。组分4截留物在血清学和免疫研究中表现得像一种完全抗原。组分4截留物和外膜制剂在血清学上相关，但它们并不相同。化学研究揭示了通过乙醇提取获得的外膜碳水化合物成分与组分4之间的相似性。用乙醇提取的外膜、组分4及其低分子量和高分子量糖蛋白含有阿拉伯糖、鼠李糖、岩藻糖、木糖、甘露糖、半乳糖、葡萄糖、氨基葡萄糖和葡萄糖醛酸。在所有这些样品的甲基糖苷的O-三甲基硅烷基衍生物的气液色谱分析中观察到三个未鉴定的峰，其中一个峰与3-O-甲基甘露糖的O-三甲基硅烷基衍生物共洗脱。
