Mallinger A G, Mallinger J, Himmelhoch J M, Neil J F, Hanin I
Psychopharmacology (Berl). 1980;68(3):249-55. doi: 10.1007/BF00428111.
In this investigation the intracellular: extracellular distribution of lithium across the erythrocyte membrane (RBC lithium distribution) was studied in vitro and in vivo. The in vitro studies were conducted by incubating cells from drug-free subjects in lithium-containing physiologic media. The in vivo studies consisted of measurements of plasma and erythrocyte (RBC) lithium and sodium concentrations during lithium carbonate treatment. Previous observations of a correlation between in vitro and in vivo RBC lithium distribution values were verified, and it was found that addition of 0.1 mM ouabain to the in vitro incubation media abolished this correlation. To examine the relationship between RBC lithium distribution and specific clinical features of depression, in vitro studies were conducted with RBCs from 20 depressed patients and 14 nondepressed control subjects. The patients were categorized as having bipolar (manic-depressive), unipolar, or secondary depressive disorders. After in vitro incubation with lithium for 48 h, the RBCs from bipolar patients had lithium distribution values that were similar to those observed in ouabain-treated cells. It appears that the lithium transport characteristics of RBCs of bipolar patients may differ from those of other depressed patients or nondepressed subjects.
在本研究中，对锂在红细胞膜内外的分布（红细胞锂分布）进行了体外和体内研究。体外研究是通过将无药物受试者的细胞在含锂的生理介质中孵育来进行的。体内研究包括在碳酸锂治疗期间测量血浆和红细胞（RBC）中的锂和钠浓度。先前关于体外和体内红细胞锂分布值之间相关性的观察得到了验证，并且发现向体外孵育介质中添加0.1 mM哇巴因消除了这种相关性。为了研究红细胞锂分布与抑郁症特定临床特征之间的关系，对20名抑郁症患者和14名非抑郁症对照受试者的红细胞进行了体外研究。患者被分类为患有双相（躁狂抑郁）、单相或继发性抑郁症。在体外与锂孵育48小时后，双相患者的红细胞锂分布值与在哇巴因处理的细胞中观察到的值相似。看来双相患者红细胞的锂转运特征可能与其他抑郁症患者或非抑郁症受试者不同。
