Beilin L J, Knight G J, Munro-Faure A D, Anderson J
J Gen Physiol. 1966 Sep;50(1):61-74. doi: 10.1085/jgp.50.1.61.
Experiments are described which indicate that iodinated human serum albumin underestimates the amount of extracellular sodium trapped in the packed layer of red blood cells, when cells and plasma are separated by centrifugation. Sucrose-(14)C also underestimates the amount of trapped extracellular sodium, but the difference between the percentages of sucrose-(14)C and extracellular sodium trapped is constant and independent of mean relative centrifugal force. It is concluded that human red blood cell sodium concentration can be measured with accuracy (a) if trapped plasma sodium is estimated with radioisotopes of sodium and a correction made for entry of sodium into the cells, providing cells and plasma can be separated rapidly; (b) by the use of sucrose as a standard plasma marker to derive the amount of trapped plasma sodium; (c) by washing the cells with sodium-free solutions. Reported values for red blood cell sodium concentration in healthy adults are critically reviewed.
本文描述了一些实验，这些实验表明，当通过离心分离细胞和血浆时，碘化人血清白蛋白会低估被困在红细胞堆积层中的细胞外钠的量。蔗糖-（14）C也会低估被困细胞外钠的量，但蔗糖-（14）C与被困细胞外钠的百分比之间的差异是恒定的，且与平均相对离心力无关。得出的结论是，如果用钠的放射性同位素估计被困血浆钠，并对进入细胞的钠进行校正，前提是细胞和血浆能够快速分离，那么可以准确测量人红细胞钠浓度；（b）通过使用蔗糖作为标准血浆标志物来推导被困血浆钠的量；（c）用无钠溶液洗涤细胞。对健康成年人红细胞钠浓度的报告值进行了严格审查。
