Almon H, Böhme H
Biochim Biophys Acta. 1980 Aug 5;592(1):113-20. doi: 10.1016/0005-2728(80)90118-8.
Heterocysts of the blue-green alga Nostoc muscorum have been isolated by prolonged treatment with lysozyme. Quantitative data are presented which show the occurrence of cytochromes c-553, f-557 and b-563 in heterocysts in amounts comparable to vegetative cells. Particularly the content of the water-soluble cytochrome c-553 can be used to evaluate the intactness of a heterocyst preparation. Cytochrome f-557 has been partially purified and found to be a c-type cytochrome corresponding to cytochrome f of higher plants and other algae. Cytochrome b-559 is present in vegetative cells but not in heterocysts. The content of plastoquinone in heterocysts is reduced to 42% of the amount present in vegetative cells. These data suggest a degradation of Photosystem II during heterocyst differentiation. Measurements of photosynthetic electron transport in heterocysts proved the inability of the photosynthetic apparatus to carry out electron transport with electrons donated by water or diphenylcarbazide. In Tris-washed thylakoids from vegetative cells, however, diphenylcarbazide can act as an electron donor to Photosystem II.
通过用溶菌酶长时间处理，已分离出蓝绿藻地木耳的异形胞。给出的定量数据表明，异形胞中细胞色素c-553、f-557和b-563的含量与营养细胞相当。特别是水溶性细胞色素c-553的含量可用于评估异形胞制剂的完整性。细胞色素f-557已被部分纯化，发现它是一种c型细胞色素，与高等植物和其他藻类的细胞色素f相对应。细胞色素b-559存在于营养细胞中，但不存在于异形胞中。异形胞中质体醌的含量降至营养细胞中含量的42%。这些数据表明在异形胞分化过程中光系统II发生了降解。对异形胞中光合电子传递的测量证明，光合装置无法利用水或二苯卡巴肼提供的电子进行电子传递。然而，在来自营养细胞的经Tris洗涤的类囊体中，二苯卡巴肼可以作为光系统II的电子供体。
