Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, D-7750, Konstanz, Germany (FRG).
Photosynth Res. 1987 Jan;11(1):37-44. doi: 10.1007/BF00117672.
The endogenous plastocyanin (PC) concentrations of Dunaliella cultures were varied from 0.3 to 3.1 molecules per pigment 700 (P700) by decreasing the Cu(+) supply of the nutrient. With these cultures the amount of PC which is sufficient for maximum photosynthesis in intact cells was determined to be about 1 to 1.5 PC/P700. Chloroplasts were also prepared from these cells and were employed in enzyme kinetic measurements of the PSI reaction from ascorbate reduced diaminodurene (DAD) to methylviologen/O2. The k m value for DAD in this reaction was 106 μM. A decrease of the endogenous PC concentration caused no change of the k m value but affected the V max in the DAD-dependent reaction. A similar interference of the PC concentration on the maximum reaction rate could also be observed when the light intensity was varied.
通过降低营养物中的 Cu(+) 供应，将杜氏盐藻培养物中的内源性质体蓝素 (PC) 浓度从 0.3 变化到 3.1 个分子每色素 700 (P700)。用这些培养物确定了在完整细胞中进行最大光合作用所需的 PC 量约为 1 到 1.5 PC/P700。还从这些细胞中制备了叶绿体，并在酶动力学测量中从抗坏血酸还原二氨基二氮杂菲 (DAD) 到甲基紫精/O2 反应的 PSI 反应中使用了叶绿体。该反应中 DAD 的 k m 值为 106 μM。内源性 PC 浓度的降低不会改变 DAD 依赖反应的 k m 值，但会影响 V max。当改变光强度时，也可以观察到 PC 浓度对最大反应速率的类似干扰。
