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用于脑膜炎奈瑟菌Y群和W135群血清群鉴别的改良抗血清琼脂方法

Improved antiserum agar method for the serogroup differentiation of Neisseria meningitidis Y and W135.

作者信息

Ashton F E, Ryan A, Diena B B

出版信息

Can J Microbiol. 1980 May;26(5):630-2. doi: 10.1139/m80-109.

DOI:10.1139/m80-109
PMID:6772290
Abstract

Rabbit antisera prepared to meningococcal serogroups Y and W135 strains were compared with horse antisera using the antiserum agar method (ASA) for the serogroup identification of Neisseria meningitidis. Thirty-seven group Y strains formed immunoprecipitates with the Y rabbit serum only, whereas the same Y strains formed immunoprecipitates with both the Y and W135 horse antisera. Forty-seven W135 strains formed specific immunoprecipitates with both the rabbit and horse W135 antisera by ASA. None of the 166 meningococcal isolates, representative of other meningococcal serogroups, formed immunoprecipitates with the groups Y and W135 rabbit or horse antisera. Use of specific Y and W135 rabbit antisera in ASA provides an improved technique for the serogroup differentiation of groups Y and W135 meningococci.

摘要

使用抗血清琼脂法(ASA)对脑膜炎奈瑟菌进行血清群鉴定,比较了针对脑膜炎球菌血清群Y和W135菌株制备的兔抗血清与马抗血清。37株血清群Y菌株仅与Y兔血清形成免疫沉淀,而相同的Y菌株与Y和W135马抗血清均形成免疫沉淀。47株W135菌株通过ASA与兔和马W135抗血清均形成特异性免疫沉淀。代表其他脑膜炎球菌血清群的166株脑膜炎球菌分离株,均未与血清群Y和W135兔或马抗血清形成免疫沉淀。在ASA中使用特异性Y和W135兔抗血清为血清群Y和W135脑膜炎球菌的血清群鉴别提供了一种改进技术。

相似文献

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Improved antiserum agar method for the serogroup differentiation of Neisseria meningitidis Y and W135.用于脑膜炎奈瑟菌Y群和W135群血清群鉴别的改良抗血清琼脂方法
Can J Microbiol. 1980 May;26(5):630-2. doi: 10.1139/m80-109.
2
Evaluation of the antiserum agar method for the serogroup identification of Neisseria meningitidis.用于脑膜炎奈瑟菌血清群鉴定的抗血清琼脂方法的评估
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Immunochemical studies and genetic background of two Neisseria meningitidis isolates expressing unusual capsule polysaccharide antigens with specificities of both serogroup Y and W135.两株表达具有Y群和W135群特异性的异常荚膜多糖抗原的脑膜炎奈瑟菌分离株的免疫化学研究及遗传背景
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Elaboration of both the group W135 and group Y capsular polysccharides by a single strain of Neisseria meningitidis.一株脑膜炎奈瑟菌对W135群和Y群荚膜多糖的合成。
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Rapid serogroup identification of Neisseria meningitidis by using antiserum agar: Prevalence of serotypes in a disease-free military population.使用抗血清琼脂对脑膜炎奈瑟菌进行血清群快速鉴定:无疾病军事人群中血清型的流行情况。
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引用本文的文献

1
Serological specificities of murine hybridoma monoclonal antibodies against Neisseria meningitidis serogroups B, C, Y, and W135 and evaluation of their usefulness as serogrouping reagents by indirect whole-cell enzyme-linked immunosorbent assay.抗B、C、Y和W135群脑膜炎奈瑟菌的鼠杂交瘤单克隆抗体的血清学特异性及其作为血清分组试剂通过间接全细胞酶联免疫吸附测定法的效用评估。
Clin Diagn Lab Immunol. 2005 Jan;12(1):152-6. doi: 10.1128/CDLI.12.1.152-156.2005.
2
Outbreak of Neisseria meningitidis, Edmonton, Alberta, Canada.加拿大艾伯塔省埃德蒙顿市脑膜炎奈瑟菌暴发。
Emerg Infect Dis. 2002 May;8(5):519-21. doi: 10.3201/eid0805.010337.
3
A new serogroup (L) of Neisseria meningitidis.
脑膜炎奈瑟菌的一个新血清群(L群)。
J Clin Microbiol. 1983 May;17(5):722-7. doi: 10.1128/jcm.17.5.722-727.1983.
4
Protection against infection with Neisseria meningitidis group B serotype 2b by passive immunization with serotype-specific monoclonal antibody.通过血清型特异性单克隆抗体被动免疫预防B群2b血清型脑膜炎奈瑟菌感染。
Infect Immun. 1985 Nov;50(2):510-6. doi: 10.1128/iai.50.2.510-516.1985.