Ashton F E, Ryan A, Diena B B, Frasch C E
Can J Microbiol. 1979 Jun;25(6):784-7. doi: 10.1139/m79-113.
The antiserum agar method (ASA), which is based on the formation of immunoprecipitates around bacterial growth on agar containing meningococcal hyperimmune horse serum, was evaluated for serogroup identification of Neisseria meningitidis. Four hundred meningococcal stains were serogrouped by ASA employing horse antisera to serogroups A, B, C, Y, W135, Z, and 29E and compared to serogroup identification by bacterial slide agglutination (BA) employing rabbit antisera. Overall, there was 95% agreement between the two methods. The ASA proved to be more accurate than BA since 15 strains which cross-reacted with Y and W135 rabbit antisera by BA were specifically serogrouped as either Y or W135 by ASA. In addition, 5 out of 75 strains which were ungroupable by BA were serogrouped as either B or 29E by ASA. Repeat serogroup identification of 100 meningococcal strains by ASA provided identical results thus showing the reproducibility of the method. The ASA is advantageous to BA since it is more reliable, utilizes standard antisera which do not have to be absorbed to remove cross-reactions, does not require the preparation of standardized bacterial antigen, and is simple to perform.
基于在含有脑膜炎球菌超免疫马血清的琼脂上细菌生长周围形成免疫沉淀物的抗血清琼脂法（ASA），用于脑膜炎奈瑟菌血清群鉴定的评估。采用针对血清群A、B、C、Y、W135、Z和29E的马抗血清，通过ASA对400株脑膜炎球菌菌株进行血清群分组，并与采用兔抗血清的细菌玻片凝集法（BA）进行血清群鉴定的结果相比较。总体而言，两种方法之间的一致性为95%。ASA被证明比BA更准确，因为15株通过BA与Y和W135兔抗血清发生交叉反应的菌株，通过ASA被明确血清群分组为Y或W135。此外，75株无法通过BA进行分组的菌株中，有5株通过ASA被血清群分组为B或29E。通过ASA对100株脑膜炎球菌菌株进行重复血清群鉴定得到了相同的结果，从而表明该方法具有可重复性。ASA比BA更具优势，因为它更可靠，使用的标准抗血清无需吸收以去除交叉反应，不需要制备标准化的细菌抗原，且操作简单。
