在限定培养基中培养的支持细胞进行长时间的雄激素结合蛋白（ABP）合成。

Prolonged ABP synthesis by Sertoli cells cultured in defined medium.

作者信息

Karl A F, Griswold M D

出版信息

Cell Biol Int Rep. 1980 Jul;4(7):669-74. doi: 10.1016/0309-1651(80)90206-4.

Abstract

Sertoli cells from 20 day old rats are stimulated to secrete ABP in cultured by a number of hormones and Vitamin A. Maximum ABP secretion in prolonged serum-free culture is seen when FSH, Testosterone, Insulin and Retinol are added to the medium. The plating conditions and type of medium used also affect the total ABP secretion. The decline of ABP secreted into medium by hormone stimulated cultures in prolonged incubations correlates with the detachment of the cells from the culture flask.

摘要

来自20日龄大鼠的支持细胞在培养中受到多种激素和维生素A的刺激而分泌雄激素结合蛋白（ABP）。当向培养基中添加促卵泡激素（FSH）、睾酮、胰岛素和视黄醇时，在长期无血清培养中可观察到ABP的最大分泌量。所用的接种条件和培养基类型也会影响ABP的总分泌量。在延长培养时间的情况下，激素刺激培养物分泌到培养基中的ABP的下降与细胞从培养瓶上脱落有关。