Karl A F, Griswold M D
Biochem J. 1980 Mar 15;186(3):1001-3. doi: 10.1042/bj1861001.
The synthesis of androgen-binding protein by cultured Sertoli cells is increased by insulin, retinol, follitropin and testosterone. Only follitropin will stimulate an increase in cyclic AMP in these cells, yet each agent individually increased the synthesis of androgen-binding protein in short-term cultures. For long-term culture of Sertoli cells follitropin, testosterone, insulin, and retinol appeared to act synergistically to prolong the ability of the cells to secrete androgen-binding protein. These are the first reported results which suggest an action of insulin and retinol directly on Sertoli cells even though the importance of both factors in male reproduction is known.
培养的支持细胞合成雄激素结合蛋白的过程会因胰岛素、视黄醇、促卵泡激素和睾酮而增加。只有促卵泡激素能刺激这些细胞中环状单磷酸腺苷(cAMP)的增加,但在短期培养中,每种试剂都能单独增加雄激素结合蛋白的合成。对于支持细胞的长期培养,促卵泡激素、睾酮、胰岛素和视黄醇似乎具有协同作用,可延长细胞分泌雄激素结合蛋白的能力。这些是首次报道的结果,表明胰岛素和视黄醇直接作用于支持细胞,尽管这两种因子在雄性生殖中的重要性是已知的。