一种用于检测血浆皮质醇的灵敏酶免疫测定法。

A sensitive enzyme immunoassay for plasma cortisol.

作者信息

Kominami G, Fujisaka I, Yamauchi A, Kono M

出版信息

Clin Chim Acta. 1980 May 9;103(3):381-91. doi: 10.1016/0009-8981(80)90158-8.

Abstract

A sensitive enzyme immunoassay for plasma cortisol has been developed, using cortisol-beta-D-galactosidase conjugate as labelled antigen and 4-methylumbelliferyl-beta-D-galactoside as fluorescence substrate. Sensitivity and precision were compared with those of radioimmunoassay with 3H-labelled cortisol and the same antiserum. The minimal detectable level for the enzyme immunoassay was 10 pg/tube or 1 microgram/dl of plasma, which makes it slightly more sensitive than the radioimmunoassay and 10 times more sensitive than previously reported enzyme immunoassays. However, the precision of the enzyme immunoassay was inferior to that of the radioimmunoassay.

摘要

已开发出一种用于血浆皮质醇的灵敏酶免疫测定法，使用皮质醇-β-D-半乳糖苷酶结合物作为标记抗原，4-甲基伞形酮基-β-D-半乳糖苷作为荧光底物。将其灵敏度和精密度与使用3H标记皮质醇和相同抗血清的放射免疫测定法进行了比较。酶免疫测定法的最低检测水平为10 pg/管或1微克/分升血浆，这使其比放射免疫测定法稍灵敏，比先前报道的酶免疫测定法灵敏10倍。然而，酶免疫测定法的精密度不如放射免疫测定法。