Bieglmayer C, Spona J, Schroeder R
Endocrinol Exp. 1980 Mar;14(1):17-25.
Different doses of progesterone, D-norgestrel and delta 15-D-norgestrel were injected subcutaneously for 7 days to female rats castrated 7 days prior to the experiments. One day after the last steroid injection a i.p. LH-RH test was performed, blood samples were collected by heart puncture and serum levels of r-LH and r-FSH were estimated by radioimmunoassay. LH-RH serum levels were determined in progestagen treated control groups by an anterior pituitary cell culture bioassay. Dose dependent inhibitory effects of steroids on LH-RH stimulated gonadotropin release were observed and a dissociation of r-LH and r-FSH serum levels was found. The modulatory influence of progestagens used in this study on LH-RH provoked gonadotropin release correlated with their biological efficiency evaluated in the Clauberg test. Progesterone, D-norgestrel and delta 15-D-norgestrel treatment had no significant effects on basal r-LH levels, but LH-RH provoked r-LH levels were higher than in the control animals. Progesterone and D-norgestrel suppressed basal as LH-RH stimulated r-FSH levels, whereas delta 15-D-norgestrel showed a complex influence on r-FSH secretion. A progestagen stimulated rise of LH-RH serum levels was found and indicated an additional effect of these hormones at the hypothalamic level. These data suggest the action of progestagens at various levels of the hypothalamus-pituitary axis.
在实验前7天对雌性大鼠进行阉割，然后皮下注射不同剂量的孕酮、炔诺孕酮和15-δ-炔诺孕酮，持续7天。在最后一次注射类固醇后一天，进行腹腔内注射促黄体生成素释放激素（LH-RH）试验，通过心脏穿刺采集血样，并通过放射免疫测定法估算血清中促黄体生成素（r-LH）和促卵泡生成素（r-FSH）的水平。通过垂体前叶细胞培养生物测定法测定孕酮处理对照组中的LH-RH血清水平。观察到类固醇对LH-RH刺激的促性腺激素释放具有剂量依赖性抑制作用，并且发现r-LH和r-FSH血清水平存在分离。本研究中使用的孕酮对LH-RH引起的促性腺激素释放的调节作用与其在Clauberg试验中评估的生物学效率相关。孕酮、炔诺孕酮和15-δ-炔诺孕酮处理对基础r-LH水平无显著影响，但LH-RH刺激后的r-LH水平高于对照动物。孕酮和炔诺孕酮抑制基础以及LH-RH刺激后的r-FSH水平，而15-δ-炔诺孕酮对r-FSH分泌表现出复杂的影响。发现孕酮刺激LH-RH血清水平升高，表明这些激素在下丘脑水平具有额外作用。这些数据提示孕酮在下丘脑-垂体轴的不同水平发挥作用。
