Lynch W H
J Bacteriol. 1980 Jul;143(1):338-42. doi: 10.1128/jb.143.1.338-342.1980.
The effects of temperature and attractants on chemotaxis in psychrotrophic Pseudomonas fluorescens were examined using the Adler capillary assay technique. Several organic acids, amino acids, and uronic acids were shown to be attractants, whereas glucose and its oxidation products, gluconate and 2-ketogluconate, elicited no detectable response. Chemotaxis toward many attractants was dependent on prior growth of the microorganism with these compounds. However, the organic acids, malate and succinate, caused strong chemotactic responses regardless of the carbon source used for growth of the bacteria. The temperature at which the cells were grown (30 or 5 degrees C) had no significant detectable effect on chemotaxis to the above attractants. The temperature at which the cells were assayed appeared to affect the rate but the extent of the chemotactic response, nor the concentration response curves. The ratios of the rate of accumulation of cells to the attractant malate were approximately 2, 4, and 1 at 30, 17, and 5 degrees C, respectively. Strong chemotactic responses were observed with cells assayed at temperatures approaching 0 degree C and appeared to be functional over a broad temperature range of 3 to 35 degrees C.
利用阿德勒毛细管测定技术研究了温度和引诱剂对嗜冷荧光假单胞菌趋化性的影响。结果表明，几种有机酸、氨基酸和糖醛酸是引诱剂，而葡萄糖及其氧化产物葡萄糖酸盐和2-酮葡萄糖酸盐未引发可检测到的反应。对许多引诱剂的趋化性取决于微生物此前是否与这些化合物一起生长。然而，无论用于细菌生长的碳源是什么，苹果酸和琥珀酸这两种有机酸都会引发强烈的趋化反应。细胞生长的温度（30℃或5℃）对上述引诱剂的趋化性没有显著的可检测影响。测定细胞时的温度似乎会影响趋化反应的速率，但不影响其程度，也不影响浓度反应曲线。在30℃、17℃和5℃时，细胞向引诱剂苹果酸积累的速率比分别约为2、4和1。在接近0℃的温度下测定细胞时观察到强烈的趋化反应，并且在3至35℃的较宽温度范围内似乎都起作用。
