Maeda K, Imae Y, Shioi J I, Oosawa F
J Bacteriol. 1976 Sep;127(3):1039-46. doi: 10.1128/jb.127.3.1039-1046.1976.
The swimming velocity of Escherichia coli at various constant temperatures was found to increase with increasing temperature. The frequency of tumbling had a peak at 34 degrees C and was very low both at 20 and at 39 degrees C. The swimming tracks near the surface of a slide glass showed curves, and the curvature increased the temperature. When the temperature of a bacterial suspension was suddenly changed, a transient change of the tumbling frequency was observed. A temperature drop induced a temporary increase in the tumbling frequency, and a quick rise of temperature, on the other hand, resulted in a temporary suppression of the tumbling. These dynamic responses to sudden changes of temperature was not observed in the smoothly swimming nonchemotactic strains bearing the mutations cheA and cheC and also in a mutant with the metF mutation under a smooth swimming condition.
研究发现，大肠杆菌在不同恒定温度下的游动速度随温度升高而增加。翻滚频率在34℃时达到峰值，在20℃和39℃时都非常低。载玻片表面附近的游动轨迹呈曲线状，且曲率随温度升高而增大。当细菌悬液的温度突然变化时，会观察到翻滚频率的瞬时变化。温度下降会导致翻滚频率暂时增加，而温度快速上升则会导致翻滚暂时受到抑制。在携带cheA和cheC突变的非趋化性平滑游动菌株以及处于平滑游动条件下的metF突变体中，未观察到对温度突然变化的这些动态反应。
