葡萄球菌产肠毒素的检测。I. 方法。

Detection of staphylococcal enterotoxigenicity. I. Methods.

作者信息

Petrás P

出版信息

J Hyg Epidemiol Microbiol Immunol. 1980;24(1):22-8.

Abstract

A culture medium has been developed, using the components easily available in Czechoslovakia, that ensures high-yield production of staphylococcal enterotoxins. Its quality was tested by the "cellophane-over-age" cultivation method using standard prototype strains of staphylococci. The microprecipitation method for the detection of staphylococcal enterotoxins was modified and practically tested. Its sensitivity was 0.3 micrograms/ml in enterotoxins A, B and C, and 0.6 micrograms/ml in types D and E.

摘要

已研发出一种培养基，其使用捷克斯洛伐克容易获取的成分，可确保高产葡萄球菌肠毒素。采用“玻璃纸覆盖老化”培养法，使用葡萄球菌标准原型菌株对其质量进行了检测。对检测葡萄球菌肠毒素的微量沉淀法进行了改进并实际测试。其对A、B和C型肠毒素的灵敏度为0.3微克/毫升，对D型和E型肠毒素的灵敏度为0.6微克/毫升。