Mott G E, Jackson E M, Morris M D
J Lipid Res. 1980 Jul;21(5):635-41.
We estimated the effects of age, sex, saturated and polyunsaturated dietary fat, and two levels of dietary cholesterol intake on cholesterol absorption in juvenile baboons between 15 and 51 months of age. We compared the reproducibility of methods of measuring cholesterol absorption within animals, and also compared estimates of percentage cholesterol absorption by three different methods. Cholesterol absorption was significantly greater in all diet groups at 27 and 39 months of age than it was at 15 or 51 months (P < 0.001). Cholesterol absorption (%) was significantly greater in animals fed low cholesterol diets than it was in animals fed high cholesterol diets. However, cholesterol absorption was not affected by type of fat (saturated or unsaturated) in the diet or by sex. The range of reproducibility (within-animal standard deviation) of percentage cholesterol absorption for different diet groups was 3.35 to 3.90% for the Borgström method (1969. J. Lipid Res. 10: 331--337), 4.67 to 7.30% for the Sodhi method (1971. Proc. Soc. Exp. Biol. Med. 137: 277-279), and 3.69 to 7.15% by a combined isotopic chromatographic method (Method I). Percentage absorption measured by either the Sodhi method or Method I was significantly greater than that measured by the Borgström method.
我们评估了年龄、性别、饱和与多不饱和膳食脂肪以及两种膳食胆固醇摄入量水平对15至51月龄幼年狒狒胆固醇吸收的影响。我们比较了动物体内测量胆固醇吸收方法的可重复性，还比较了三种不同方法对胆固醇吸收百分比的估计。在27和39月龄时，所有饮食组的胆固醇吸收均显著高于15或51月龄时（P < 0.001）。喂食低胆固醇饮食的动物的胆固醇吸收（%）显著高于喂食高胆固醇饮食的动物。然而，胆固醇吸收不受饮食中脂肪类型（饱和或不饱和）或性别的影响。不同饮食组胆固醇吸收百分比的可重复性范围（动物体内标准差），采用Borgström方法（1969年。《脂质研究杂志》10：331 - 337）为3.35%至3.90%，采用Sodhi方法（1971年。《实验生物学与医学学会会刊》137：277 - 279）为4.67%至7.30%，采用同位素色谱联合方法（方法I）为3.69%至7.15%。采用Sodhi方法或方法I测得的吸收百分比显著高于采用Borgström方法测得的吸收百分比。
