Crouse J R, Grundy S M
J Lipid Res. 1978 Nov;19(8):967-71.
A new method has been developed for measurement of cholesterol absorption. Seven patients were fed [14C]cholesterol together with [3H]-beta-sitosterol three times daily for 10 days. Stools were collected on days 3--10. The ratio of isotopes in stools became essentially constant after the first 3 days of isotope administration. Percentage absorption of cholesterol was calculated from the difference between the dietary and fecal isotope ratios. Percentage absorption of cholesterol in these seven patients ranged from 47 to 65% (mean 54%). The coefficient of variation on individual samples for all the patients ranged from 3.2 to 15.0% (mean 7.8%). This method was evaluated in five additional patients by comparison with a standard sterol balance method (Method II, see Quintao, E., S. M. Grundy, and E. H. Ahrens. 1971. J. Lipid Res. 12: 221--232). The results obtained by the two methods were essentially identical. The new method offers the advantages of simplicity of administration of isotope, ease of analysis of stool samples, and daily repeatability of analysis.
已开发出一种用于测量胆固醇吸收的新方法。七名患者每天三次服用[14C]胆固醇和[3H]-β-谷甾醇，持续10天。在第3至10天收集粪便。在给予同位素的前3天后，粪便中同位素的比例基本保持恒定。根据饮食和粪便同位素比例的差异计算胆固醇的吸收百分比。这七名患者中胆固醇的吸收百分比在47%至65%之间（平均54%）。所有患者个体样本的变异系数在3.2%至15.0%之间（平均7.8%）。通过与标准甾醇平衡法（方法II，见昆陶，E.，S.M. 格伦迪和E.H. 阿伦斯。1971年。《脂质研究杂志》12: 221 - 232）比较，对另外五名患者评估了该方法。两种方法得到的结果基本相同。新方法具有同位素给药简单、粪便样本分析容易以及分析可每日重复的优点。
