Abdurakhmanov I A, Erokhin Iu E
Mol Biol (Mosk). 1980 May-Jun;14(3):539-48.
Linear dichroism and orientation of pigments in chromatophores of photosynthetic bacteria Chromatium minutissimum and Rhodospirillum rubrum using a novel method of orientation in polyacrylamide gel was studied. A model is proposed for orientation of spherical membranes of chromatophores or other similar vesicules. The value of linear dichroism is derived for known deformation of the gel and a certain angle between the transition dipole and a unit vector perpendicular to the membrane plane. The analysis of linear dichroism spectra permits calculation of angles between the normal to the membrane and the transition dipoles in Chr. minutissimum 65 degrees +/- 1.5 degrees (890 nm absorption band), 63 degrees +/- 1 degree (860 nm), 63 degrees +/- 1 degree (800 nm), 45.5 degrees +/- 1 degree (590 nm), 50.5 degrees +/- 0.5 degree (450--550 nm) and in Rsp. rubrum: 71 degrees +/- 1.5 degree (890 nm), 66.5 degrees +/- 1 degree (870 nm), 69 degrees +/- 1.5 degree (800 nm), 37 degrees +/- 0.5 degree (590 nm), 49.5 degrees +/- 0.5 degree (450--550 nm). The 860 nm band shift to shorter wave-lengths observed in Chr. minutissimum chromatophores treated with 0.01 M potassium ferricyanide is not related to reorientation of transition dipoles, but rather to certain changes of lipid-protein environment.
利用一种在聚丙烯酰胺凝胶中进行取向的新方法，研究了光合细菌极小色杆菌和深红红螺菌的色素在载色体中的线性二色性和取向。提出了一个关于载色体或其他类似囊泡的球形膜取向的模型。对于已知的凝胶变形以及跃迁偶极矩与垂直于膜平面的单位向量之间的特定角度，推导出了线性二色性的值。对线性二色性光谱的分析允许计算极小色杆菌中膜法线与跃迁偶极矩之间的角度：65度±1.5度（890纳米吸收带）、63度±1度（860纳米）、63度±1度（800纳米）、45.5度±1度（590纳米）、50.5度±0.5度（450 - 550纳米）；以及深红红螺菌中的角度：71度±1.5度（890纳米）、66.5度±1度（870纳米）、69度±1.5度（800纳米）、37度±0.5度（590纳米）、49.5度±0.5度（450 - 550纳米）。在用0.01 M铁氰化钾处理的极小色杆菌载色体中观察到的860纳米波段向较短波长的移动，与跃迁偶极矩的重新取向无关, 而是与脂 - 蛋白环境的某些变化有关。
