[Analysis of the linear dichroism of reaction centers oriented in polyacrylamide gel].

A model for orientation of rigid disc-shaped and rod-shaped macromolecules in polymer is analysed. Analytical expressions are given for the dependence of linear dichroism value on the angle between the transition dipole vector and the axis of the macromolecules, for certain deformation of the sample. The reaction centers from Rhodopseudomonas sphaeroides R-26 are oriented as rod-shaped particles. The angles between the long axis of the reaction center and transition dipoles for bacteriochlorophyll 31.8 +/- 2,4 degrees (870 nm), 42,3 +/- 0.9 degrees (796 nm), 68.0 +/- 0.6 degrees (600 nm) and for bacteriopheophytin 76.5 +/- 1.9 degrees (760 nm), 39.8 +/- 1.5 degrees (540 nm) are determined from absorption spectra.