信号序列不足以引导β-半乳糖苷酶离开细胞质。
A signal sequence is not sufficient to lead beta-galactosidase out of the cytoplasm.
作者信息
Moreno F, Fowler A V, Hall M, Silhavy T J, Zabin I, Schwartz M
出版信息
Nature. 1980 Jul 24;286(5771):356-9. doi: 10.1038/286356a0.
DOI:10.1038/286356a0
PMID:6772962
Abstract
Escherichia coli strains have been constructed in which lacZ, the gene for the cytoplasmic enzyme beta-galactosidase, is fused to lamB, the gene for an outer membrane protein. One such strain produces a beta-galactosidase which remains cytoplasmic even though it possesses the complete signal sequence of the lamB protein precursor at the amino-terminal end.
摘要
已经构建了大肠杆菌菌株，其中编码胞质酶β-半乳糖苷酶的基因lacZ与编码外膜蛋白的基因lamB融合。一种这样的菌株产生的β-半乳糖苷酶尽管在氨基末端具有lamB蛋白前体的完整信号序列，但仍保留在细胞质中。
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