Simmons R M, Szent-Györgyi A G
Nature. 1980 Aug 7;286(5773):626-8. doi: 10.1038/286626a0.
We have shown previously that chemically skinned fibre bundles from scallop muscle can be desensitized to the action of calcium by the removal of the regulatory light chains of myosin and that sensitivity can be restored by the re-addition of scallop light chains. We have now confirmed these results and extended our observations to fibre bundles from which one or both of the regulatory light chains per myosin have been removed (by treatment with EDTA at 7 and 25 degrees C, respectively) and replaced by the corresponding light chains from other species. In the case of a double substitution--where both light chains were replaced--sensitivity was restored completely by light chains from other molluscs and from gizzard muscle; for a single substitution there was restoration of sensitivity by all the light chains tested.
我们之前已经表明，通过去除肌球蛋白的调节轻链，扇贝肌肉的化学去皮纤维束可对钙的作用产生脱敏，并且通过重新添加扇贝轻链可恢复敏感性。我们现在已经证实了这些结果，并将我们的观察扩展到了这样的纤维束，其中每个肌球蛋白的一个或两个调节轻链已被去除（分别在7摄氏度和25摄氏度下用EDTA处理），并被来自其他物种的相应轻链所取代。在双重取代的情况下——即两个轻链都被取代——来自其他软体动物和砂囊肌肉的轻链可完全恢复敏感性；对于单一取代，所有测试的轻链都能恢复敏感性。
