Brockerhoff H, Wisniewski H M, Lipton L C, Deshmukh D S
Neurochem Res. 1980 Jun;5(6):617-28. doi: 10.1007/BF00964783.
We describe an attempt to incorporate a metabolically inert phospholipid analog into animal membranes, especially myelin, in vivo, with the view of eventual long-term membrane modification or membrane engineering. A sonicated suspension of a mixture of [14C]phosphatidylcholine and its dialkyl analog, [3H] tetradecyloctadecano(1)phosphocholine, was injected into the brain of weanling rats. Samples were counted of whole brain, myelin, liver, and carcass, at intervals from 1 to 63 days, and the composition of the extracted lipid was determined by thin-layer chromatography. Both lipid labels were found to be cleared from the body at similar rates, but while phosphatidylcholine was metabolized within a day, with the label appearing mainly in the phosphatidylethanolamine fraction and in nonpolar lipids, the dialkylphosphatidylcholine remained intact, with retention in myelin of a small but almost constant amount for a month. Ways will have to be found to enhance uptake of the lipids by the brain.
我们描述了一种尝试，即在体内将代谢惰性的磷脂类似物掺入动物膜，尤其是髓磷脂中，以期最终实现长期的膜修饰或膜工程。将[14C]磷脂酰胆碱及其二烷基类似物[3H]十四烷基十八烷（1）磷酰胆碱的混合物的超声悬浮液注入断奶大鼠的大脑。在1至63天的间隔内，对全脑、髓磷脂、肝脏和胴体进行取样计数，并通过薄层色谱法测定提取脂质的组成。发现两种脂质标记物从体内清除的速率相似，但磷脂酰胆碱在一天内就被代谢，标记物主要出现在磷脂酰乙醇胺部分和非极性脂质中，而二烷基磷脂酰胆碱保持完整，在髓磷脂中保留少量但几乎恒定的量长达一个月。必须找到提高大脑对脂质摄取的方法。
