Jauker F
Biochem J. 1980 Apr 1;187(1):141-6. doi: 10.1042/bj1870141.
A three-parameter model was used to test a curvilinear regression for its gamma-intercept. It was applied in the kinetic analysis of a model reaction and of real experiments. The model reaction consisted of a dialysis experiment with increasing amounts of background added at t0. In the presence of this background, initial rates of solute exchange were determined precisely enough to be in agreement with Fick's law of diffusion. The biological experiments were concerned with the saturation kinetics of the acid-soluble radioactivity of cells exposed to 3H-labelled amino acids. Two models of regression were applied, facilitated and physical diffusion, both consistent with the assumption of a substantial amount of background. This background was assumed to be caused by adsorption of 3H-labelled amino acids to cell-surface proteins. Without further consideration it caused a systematic overestimation of the initial rates.
使用一个三参数模型来测试其γ截距的曲线回归。它被应用于模型反应和实际实验的动力学分析中。模型反应包括一个透析实验，在t0时刻添加越来越多的背景物质。在这种背景存在的情况下，溶质交换的初始速率被精确测定，足以与菲克扩散定律相符。生物学实验涉及暴露于3H标记氨基酸的细胞中酸溶性放射性的饱和动力学。应用了两种回归模型，促进扩散和物理扩散，两者都与大量背景的假设一致。这种背景被认为是由3H标记氨基酸吸附到细胞表面蛋白质上引起的。未经进一步考虑，它导致了对初始速率的系统性高估。
