梨形四膜虫无细胞蛋白质合成提取物的制备及部分特性分析
Preparation and partial characterization of cell-free protein-synthesizing extracts from Tetrahymena pyriformis.
作者信息
David E T, Smith K E
出版信息
Biochem J. 1981 Mar 15;194(3):761-70. doi: 10.1042/bj1940761.
Abstract
- We have examined methods necessary for preparing post-mitochondrial supernatants from Tetrahymena pyriformis strain HSM that are capable of efficient cell-free protein synthesis. 2. The requirements for optimum synthesis in these extracts are described. 3. Data relating to the kinetics of protein synthesis and the initiation capacity of these supernatants are presented.
摘要
- 我们已经研究了从嗜热四膜虫HSM菌株制备线粒体后上清液的必要方法，这些上清液能够进行高效的无细胞蛋白质合成。2. 描述了这些提取物中最佳合成的要求。3. 给出了与蛋白质合成动力学以及这些上清液的起始能力相关的数据。
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