生物流体中丙吡胺及其[13C, 15N]标记类似物的稳定同位素稀释测定法。

A stable isotope dilution assay for disopyramide and its [13C, 15N]labelled analogue in biological fluids.

作者信息

Haskins N J, Ford G C, Palmer R F, Waddell K A

出版信息

Biomed Mass Spectrom. 1980 Feb;7(2):74-9. doi: 10.1002/bms.1200070207.

DOI:10.1002/bms.1200070207

Abstract

The mass spectra of disopyramide phosphate and two stable isotopically labelled analogues have been obtained using electron impact and chemical ionization. The low isotopic purity of [13C, 15N)disopyramide phosphate was shown to be due to the low isotopic purity of the 15N label. A stable isotope dilution assay for disopyramide and [13C, 15N]disopyramide in biological fluids has been developed using [2H14]disopyramide phosphate as the internal standard. This assay will be used to analyse samples obtained after the co-administration of disopyramide phosphate intravenously and [13C, 15N]disopyramide phosphate orally to several animal species.

摘要

使用电子轰击和化学电离法获得了磷酸丙吡胺及其两种稳定同位素标记类似物的质谱图。结果表明，[13C, 15N]磷酸丙吡胺的低同位素纯度是由于15N标记的低同位素纯度所致。以[2H14]磷酸丙吡胺为内标，建立了生物体液中丙吡胺和[13C, 15N]丙吡胺的稳定同位素稀释分析法。该分析法将用于分析几种动物静脉注射磷酸丙吡胺和口服[13C, 15N]磷酸丙吡胺后获得的样本。